DepartmentCellular and Integrative Physiology
Qian Shi, Ph.D.
Manzoor Bhat, M.S., Ph.D.
My research career thus far has spanned in vivo whole animal studies to cell and molecular biology in mammalian systems. My training also encompasses experimental and computational biology. My research as a graduate student examined the role of gap junction protein Connexin in regulating lens development and promoting lens differentiation. During this period, I acquired a broad background in Biochemistry and Molecular Biology, as well as membrane cell biology. I also independently carried out computational structural simulation of important membrane proteins, which strengthened the experimental findings in my previous research. In my previous postdoc research, I focused on studying the role of ROS/NAPDH oxidase and related signaling pathways during development of cancer and diabetes. I use an in vitro primary cells model that permits manipulation of various gene expression and examination of signaling pathways involved in pathogenesis of the disease. In vivo cancer mouse model is also used in my research to elucidate further the mechanisms by which/how diabetes/cancer enhances ROS and Nox protein expression and related mechanism. Joining Dr. Bhat’s Lab since 2016, I am currently focusing on mechanistic studies of pathogenesis of myelination and node of Ranvier in a rare multisystem genetic disorder, tuberous sclerosis complex (TSC). Using genetic modified mouse models, I am trying to reveal the molecular mechanism that leads to defects in the myelinated axon domain organization as is often observed in TSC patients.
Awards & Accomplishments
Awards and Honors
Mar. 2016 Advanced Postdoctoral Fellowship Award ($270,000 for 2016-2019), Juvenile Diabetes Research Foundation (JDRF)
May 2016 Best Poster Awards for Postdoc/Student Research forum Department of Physiology, UTHSCSA
Nov 2015 Travel Award to Advances in Research of ASN Annual Meeting American Society of Nephrology
Mar. 2013 Postdoctoral Fellowship Award ($148,296 for 2013-2016), Juvenile Diabetes Research Foundation (JDRF)
May 2013 Trainee Winner Award for Research Day Department of Medicine, UTHSCSA
Dec. 2010 Pre-doctoral Travel Award American Society for Cell Biology 50th Annual Meeting
Invited talk at JDRF Training Grantees Meeting, New York, Nov. 2017
Title: Novel therapeutic treatment of diabetic nephropathy.
Invited talk at Huazhong University of Science and Technology, China. Dec. 2015
Title: The role of NOX4 in pathogenesis development of tuberous sclerosis complex and diabetic nephropathy.
Poster Presentation at 2015 American Society for Cell Biology Annual Meeting
Title: NADPH Oxidase 4 as a novel therapeutic target to treat Tuberous Sclerosis Complex.
Oral presentation in 2013 Cold Spring Harbor Asia Conferences (Cell Signaling in Metabolism, Inflammation & Cancer), Suzhou, China
Title: NADPH oxidase 4 Expression is up-Regulated in Tuberin Deficiency through mTOR: Implication for the Treatment of Tuberous Sclerosis Complex
Poster Presentation at 2010 American Society for Cell Biology50th Annual Meeting
Title: Cx50 Regulates Lens Differentiation via the Interaction with S Phase Kinase Protein 2
Oral presentation at 2009 International Gap Junction Conference
Title: Opening of Connexin 43 Hemichannels is regulated through the Interaction with Integrin a5/b1 in Response to Mechanical Stimulation.
Poster Presentation at 2009 International Gap Junction Conference
Title: An Amino Acid Residue at the C-terminus of Connexin 50 Plays a Crucial Rolein Lens Epithelial-Fiber Cell Differentiation.
Q. Shi, S. Viswanadhapalli, W. E. Friedrichs, C. Velagapudi, C. Szyndralewiez, S. Bansal, M.A. Bhat, G.G. Choudhury, and H. E. Abboud. Nox4 is a Target for Tuberin Deficiency Syndrome. Sci. Rep. 2018 doi: 10.1038/s41598-018-21838-4
Niu*, Q. Shi*, W. Zhang, Y. Shu, N. Yang, B, Chen, Q. Wang, X. Zhao, J. Chen, N. Cheng, X. Feng, J. Ji and P. Shen. Caspase-1 cleaves PPARγ for potentiating the pro-tumor action of TAMs. Nat. Comm. 2017, 3;8(1):766 * Equal contribution
Y. Yao*, Q. Shi*, B. Chen, Q. Wang, X. Li, L. Li, Y. Huang, J. Ji and P. Shen. Identification of caspase-6 as a new regulator of alternatively activated macrophages. J. Bio. Chem. 2016, 291 (33): 17450. * Equal contribution.
Q. Shi and J. X. Jiang. Connexin Arrests Cell Cycle Through Cytosolic Retention of an E3 Ligase. Mol. & Cell. Onco. 2016 Feb 18;3(2): e1132119.
Q.D. Zhao, S. Viswanadhapalli, P. Williams, Q. Shi, C. Tan, X. Yi, B. Bhandari, H.E. Abboud. NADPH Oxidase 4 Induces Cardiac Fibrosis and Hypertrophy Through Activating Akt/mTOR and NFκB Signaling Pathways. Circulation. 2015, 131(7): 643-655
Q. Shi, X. S. Yu, T. W. White, E.A. Bank, S. Gu and J. X. Jiang. Connexin Controls Cell-Cycle Exit and Cell Differentiation by Directly Promoting Cytosolic Localization and Degradation of E3 Ligase Skp2. Dev. Cell. 2015, 35(4):483-496.
Q. Shi, R. Padmanabhan, C. J. Villegas, S. Gu, and J. X. Jiang. Membrane Topological Structure of System N/A Neural Amino Acid Transporter SNAT4. J. Biol Chem.2011, Sep 14. 286: 38086-38094.
Q. Shi, E.A. Banks, X.S. Yu, S. Gu, J. Lauer, G.B. Fields, J.X. Jiang. Amino acid residue V362 plays a critical role in maintaining the structure of C-terminus of connexin 50 and in lens epithelial-fiber differentiation. J Biol Chem. 2010, 285(24):18415-18422.
T. Chen, Q. S. Wang, J. Cui, W. Yang, Q. Shi, J. G. Ji, P. P. Shen, Induction of apoptosis in mice liver by microcystin: a combined transcriptomic, proteomic and simulation strategy. Molecular & Cell Proteomics, 2005 (4):958-974. (Cover story)
Q. Shi, J. Cui, J. Zhang, F. X. Kong, Z. C. Hua, P. P. Shen, Expression modulation of multiple cytokines in vivo by cyanobacteria blooms extract from Taihu Lake, China. Toxicon 2004 (44):871-879.