DepartmentCellular and Integrative Physiology
Nina Boiko, Ph.D.
James Stockand, Ph.D.
B.S., M.S. - National Technical University of Ukraine, 2000, 2001
Ph.D. - Bogomoletz Institute of Physiology at the Ukrainian National Academy of Sciences, 2005
My research is focused on the cellular and physiological function of Degenerin/ENaC ion channels. I am currently involved in studies of structure-function and regulation of the epithelial Na+ channel (ENaC), known to be involved in epithelial ion transport, and studies of related acid-sensing ion channel 5 (ASIC5) in the regulation of neuronal activity in the brain. My primary effort to date has been study of Degenerin/ENaC channels in fly sensory neurons with a focus on understanding the cellular function of these channels and their contribution to the ionic basis of sensory transduction and pain. This combined with recent advances in ion channel recording in fly sensory neurons, as pioneered by me, allows sensory function to be probed in parallel at the protein, cellular and whole animal levels.
I also have initiated a new project to investigate why some critical anti-cancer drugs that are widely used in the clinic cause pain. These side effects of anticancer drugs are little understood and limit the effective use of these key drugs. My research in this area has provided key understanding of the fundamental mechanisms that underlie chemotherapy induced pain.
Boiko N, Medrano G, Montano E, Jiang N, Williams CR, Madungwe NB, Bopassa JC, Kim CC, Parrish JZ, Hargreaves KM, Stockand JD, Eaton BA. TrpA1 activation in peripheral sensory neurons underlies the ionic basis of pain hypersensitivity in response to vinca alkaloids. PLoS One 12(10):e0186888, 2017
Boiko N, Berman J, Mironova E, Stockand JD. Loop diuretics are K+-sparing in the presence of a low-Na+, high-K+ diet. Kidney Int. 92(4):786-787, 2017
Boiko N, Kucher V, Stockand JD. Pseudohypoaldosteronism type 1 and Liddle’s syndrome mutations that affect the single-channel properties of the epithelial Na+ channel. Physiol Rep., Nov;3(11), 2015
Bradley Akitake, Qiuting Ren, Nina Boiko, Jinfei Ni, Takaaki Sokabe, James D. Stockand, Benjamin A. Eaton and Craig Montell. Coordination and Fine Motor Control Depends on Drosophila TRPγ. Nature Communications. 6:7288, 2015.
Mironova E, Boiko N, Bugaj V, Kucher V, Stockand JD. Regulation of Na+ excretion and arterial blood pressure by purinergic signalling intrinsic to the distal nephron: consequences and mechanisms. Acta Physiol (Oxf) 213(1):213-21, 2015.
Boiko N, Kucher V, Wang B, Stockand JD. Restrictive expression of acid-sensing ion channel 5 (asic5) in unipolar brush cells of the vestibulocerebellum. PLoS One 9(3):e91326, 2014.
Chimote AA, Hajdu P, Kucher V, Boiko N, Kuras Z, Szilagyi O, Yun YH, Conforti L. Selective inhibition of KCa3.1 channels mediates adenosine regulation of the motility of human T cells. J Immunol. 191(12):6273-80, 2013.
Nina Boiko, Volodymyr Kucher, Benjamin A. Eaton, and James D. Stockand. Inhibition of Neuronal Deg/ENaC Channels by the Multiple Sclerosis Drug 4-Aminopyridine. J Biol Chem. 288(13):9418-27, 2013.
Volodymyr Kucher, Benjamin A. Eaton, James D. Stockand, and Nina Boiko. Patch clamping Drosophila sensory neurons. Ion channels. Methods in Molecular Biology, 998:385-397, 2013
Nina Boiko, Volodymyr Kucher, James D. Stockand, and Benjamin A. Eaton. Pickpocket1 is an ionotropic molecular sensory transducer. J Biol Chem. 287(47):39878-86, 2012.
Kucher V, Boiko N, Pochynyuk O, Stockand JD. Voltage-dependent gating underlies loss of ENaC function in Pseudohypoaldosteronism type 1. Biophys. J., 100(8):1930-9, 2011.
Pochynyuk O, Kucher V, Boiko N, Mironova E, Staruschenko A, Karpushev AV, Tong Q, Hendron E, Stockand J. Intrinsic voltage dependence of the epithelial Na+ channel is masked by a conserved transmembrane domain tryptophan. J Biol Chem., 284(38):25512-21, 2009
N.Y. Boiko, N.Kh. Pogorelaya, V.V. Kucher. Allosteric influence of [K+]out changes on the gates of voltage-gated potassium channels. Reports of NAS of Ukraine, 11:150-154, 2005 [Article in Ukrainian]
I.S. Magura, V.V. Kucher, N.Y. Boiko. Voltage-gated potassium channels and their activity regulation mechanisms. Neurophysiology, 36(4):322-329, 2004 [Article in Ukrainian]
N.Y. Boiko, V.V. Kucher, N.Kh. Pogorelaya, I.S. Magura. Changes in extracellular K+ concentration modulate properties of voltage dependent K+ channel conductance in PC-12 cells. Physiology J., 50(4):57-61, 2004 [Article in Ukrainian]