DepartmentCellular and Integrative Physiology
Chase Carver, Ph.D.
Mark S. Shapiro, Ph.D.
Dr. Carver joined the Department of Physiology at the UTHSCSA as a postdoctoral researcher under the supervision of Dr. Mark S. Shapiro in Fall 2015. His focus has been to study voltage-gated ion channel physiology and correlated neurological dysfunction in determining heretofore unknown mechanisms of epileptogenesis in the hippocampus. The aim of his current research is to investigate “M-type” (KCNQ) K+ channels in hyperexcitability, since KCNQ2/3 channels are responsible for M-current to maintain homeostatic control over the neuronal resting membrane potential and firing frequency. Dr. Carver is currently investigating the interaction of muscarinic acetylcholilne receptors and M-channels in the dentate gyrus using electrophysiology and super resolution microscopy. He formed a hypothesis that in dentate gyrus granule cells, increased muscarinic actylcholine receptor activity at the axon initial segment
results in greater seizure susceptibility via depression of M-current. This study will be tested by molecular expression, neuronal imaging, electrophysiology, and behavioral studies in mice. He is using novel transgenic tools in order to investigate mechanistic modulation of activities that originate in the dentate gyrus.
In the above studies, Dr. Carver is receiving training in new technical expertise as follows:
- Total Internal Reflective Fluorescence Microscopy and Imaging
- Super Resolution STORM Imaging
- Immunocytochemistry and Immunohistochemistry using heterologous expression systems
- DREADD (Drug Receptor Exclusively Activated by Designer Drug) delivery in vivo for molecular expression and pharmacological assays
Awards & Accomplishments
2015 American Epilepsy Society Postdoctoral Fellow, American Epilepsy Society
2015 San Antonio Postdoctoral Research Forum, UTHSCSA, San Antonio, TX 2015 American Epilepsy Society, Annual Meeting, Philadelphia, PA
2016 San Antonio Postdoctoral Research Forum, UTHSCSA, San Antonio, TX
September 2015 Perimenstrual Upregulation of δ-subunit GABAA Receptors Mediating Tonic Inhibition and Neurosteroid Sensitivity. San Antonio Postdoctoral Research Forum. UTHSCSA
December 2015 Role of Tonic and M Currents in the Hippocampal Dentate Gyrus in Controlling Neuronal Excitability and Seizure Susceptibility. American Epilepsy Society, Annual Meeting, Philadelphia, PA (platform presentation) Carver CM, Shapiro MS. Parsing the necessary network components of the hippocampus in temporal lobe epileptogenesis. 5th Annual Cellular and Integrative Physiology Graduate Student Symposium. San Antonio, TX, April 7, 2017. Poster Presentation.
April 7, 2017 Carver CM, Shapiro MS. Parsing the necessary network components of the hippocampus in temporal lobe epileptogenesis. 5th Annual Cellular and Integrative Physiology Graduate Student Symposium. San Antonio, TX,. Oral Presentation.
April 7, 2017 Cook ME, Carver CM, Shapiro MS. M-type potassium channels are upregulated in the hippocampus in response to seizure activity. 5th Annual Cellular and Integrative Physiology Graduate Student Symposium. San Antonio, TX, April 7, 2017. Poster Presentation.
December, 2016 Carver CM, Shapiro MS. Maladaptive suppression of KCNQ/M-channel current by muscarinic acetylcholine receptor activation promotes epileptiform activity originating in the dentate gyrus. American Epilepsy Society Annual Meeting, Houston, TX,. Poster Presentation
September, 2016 Carver CM, Shapiro MS. Selective Activation of Muscarinic Receptors in Dentate Gyrus-CA3 Promotes Hyperexcitability and Seizure Susceptibility. Society of General Physiologists, Woods Hole, MA, Poster Presentation