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Department

Cellular and Integrative Physiology

Chase Carver, Ph.D.

Postdoctoral Fellow

Personal Statement:

Lab Affiliation:
Mark S. Shapiro, Ph.D.


Research

Dr. Carver joined the Department of Physiology at the UTHSCSA as a postdoctoral researcher under the supervision of Dr. Mark S. Shapiro in Fall 2015. His focus has been to study voltage-gated ion channel physiology and correlated neurological dysfunction in determining heretofore unknown mechanisms of epileptogenesis in the hippocampus. The aim of his current research is to investigate “M-type” (KCNQ) K+ channels in hyperexcitability, since KCNQ2/3 channels are responsible for M-current to maintain homeostatic control over the neuronal resting membrane potential and firing frequency. Dr. Carver is currently investigating the interaction of muscarinic acetylcholilne receptors and M-channels in the dentate gyrus using electrophysiology and super resolution microscopy. He formed a hypothesis that in dentate gyrus granule cells, increased  muscarinic  actylcholine  receptor  activity  at  the  axon  initial  segment

results in greater seizure susceptibility via depression of M-current. This study will be tested by molecular expression, neuronal imaging, electrophysiology, and behavioral studies in mice. He is using novel transgenic tools in order to investigate mechanistic modulation of activities that originate in the dentate gyrus.

 

In the above studies, Dr. Carver is receiving training in new technical expertise as follows:

  • Total Internal Reflective Fluorescence Microscopy and Imaging
  • Super Resolution STORM Imaging
  • Immunocytochemistry and Immunohistochemistry using heterologous expression systems
  • DREADD (Drug Receptor Exclusively Activated by Designer Drug) delivery in vivo for molecular expression and pharmacological assays

Awards & Accomplishments

2015       American Epilepsy Society Postdoctoral Fellow, American Epilepsy Society

News

Meetings Attended/Planned:

2015    San Antonio Postdoctoral Research Forum, UTHSCSA, San Antonio, TX 2015  American Epilepsy Society, Annual Meeting, Philadelphia, PA

2016     San Antonio Postdoctoral Research Forum, UTHSCSA, San Antonio, TX

Publications

September 2015  Perimenstrual Upregulation of δ-subunit GABAA Receptors Mediating Tonic Inhibition and Neurosteroid Sensitivity. San Antonio Postdoctoral                                   Research Forum. UTHSCSA

December 2015    Role of Tonic and M Currents in the Hippocampal Dentate Gyrus in Controlling Neuronal Excitability and Seizure Susceptibility. American                                       Epilepsy Society, Annual Meeting, Philadelphia, PA (platform presentation) Carver CM, Shapiro MS. Parsing the necessary network                                                 components of the hippocampus in temporal lobe epileptogenesis. 5th Annual Cellular and Integrative Physiology Graduate Student                                               Symposium. San Antonio, TX, April 7, 2017. Poster Presentation.

April 7, 2017        Carver CM, Shapiro MS. Parsing the necessary network components of the hippocampus in temporal lobe epileptogenesis. 5th Annual Cellular                              and Integrative Physiology Graduate Student Symposium. San Antonio, TX,. Oral Presentation.

April 7, 2017        Cook ME, Carver CM, Shapiro MS. M-type potassium channels are upregulated in the hippocampus in response to seizure activity. 5th Annual                                Cellular and Integrative Physiology Graduate Student Symposium. San Antonio, TX, April 7, 2017. Poster Presentation.

December, 2016  Carver CM, Shapiro MS. Maladaptive suppression of KCNQ/M-channel current by muscarinic acetylcholine receptor activation                                                         promotes epileptiform activity originating in the dentate gyrus. American Epilepsy Society Annual Meeting, Houston, TX,. Poster Presentation

September, 2016 Carver CM, Shapiro MS. Selective Activation of Muscarinic Receptors in Dentate Gyrus-CA3 Promotes Hyperexcitability and Seizure                                               Susceptibility. Society of General Physiologists, Woods Hole, MA, Poster Presentation